Fiona Hollinshead

Associate Professor Clinical Sciences

B217 VTH

(970) 297-4023

About Fiona

Dr. Fiona Hollinshead attended the University of Sydney, Faculty of Veterinary Science, Australia. She graduated in 1996 with a Bachelor of Veterinary Science (Honors) and in 2004 with a PhD in sperm sexing entitled, "Flow cytometric sorting of ram spermatozoa: Production of offspring of a pre-determined sex using in vivo and in vitro fertilisation." After completion of a two-year clinical residency in Theriogenology at Cornell University, New York, she became a Diplomate of the American College of Theriogenologists and a Registered Specialist in Small Animal Reproduction with the Australasian Veterinary Boards Council in 2007. Prior to joining CSU, Dr. Hollinshead spent the previous 12 years running a busy small animal reproduction referral practice in New Zealand and had the privilege to be the primary reproductive consultant to the NZ Guide Dogs, NZ Assistance and Mobility Dogs, MPI Detector Dogs, and NZ Police Dog breeding programs. Dr. Hollinshead has also enjoyed working with a number of breeding programs at the Hamilton and Auckland Zoos, including the AI and semen preservation of a Thai fishing cat. She is a member of the Theriogenology Foundation committee and has trained many residents for both the ACT and European board specialization in small and large animal reproduction. Dr. Hollinshead has three boys (William, Harry and Oscar) who were not intended to be named after the royal family.


BVSc (Hons), University of Sydney, Australia, 1996PhD, University of Sydney, Australia, 2004


Professional: Diplomate American College of Theriogenologists


Riley E. Thompson, Gerrit J. Bouma and Fiona K. Hollinshead, 2022 The roles of extracellular vesicles and organoid models in female reproductive physiology. Int. J. Mol. Sci. 2022, 23, 3186. Kelsey Martin, Agata Parsons, Greg Burns, Gerrit J. Bouma, Fiona Hollinshead, 2022 A unique case of SRY-negative XX sex reversal in a mixed breed dog: How to clinically approach cases with anatomical sexual disorders: Clinical Theriogenology 14, 110-114. Riley E. Thompson, Melinda A. Meyers, Budhan S. Pukazhenthi and Fiona K. Hollinshead, 2021 Evaluation of growth, viability, and structural integrity of equine endometrial organoids following cryopreservation. Cryobiology 104; 56-62 PMID: 34788682 DOI: 10.1016/j.cryobiol.2021.11.003Hollinshead F.K. and Hanlon D.W. (2018) Normal progesterone profiles during estrus in the bitch: A prospective analysis of 1420 estrous cycles. Theriogenology 125, 37-42M. Ontiveros, D. Hanlon, F. Hollinshead (2018) Seminomas and an Interstitial Cell Tumor in an 8 year old male Husky. Clinical Theriogenology 10; 2, 97-106Hollinshead F.K. and Hanlon D.W. (2017) Factors affecting the reproductive performance of bitches: A prospective cohort study involving 1203 inseminations with fresh and frozen semen. Theriogenology 101, 62-72Hyatt J, Jarratt G, Hollinshead F and Hanlon DW. (2017) The effect of early postpartum intervention on the reproductive performance of anovulatory anestrus New Zealand dairy cows. Clinical Theriogenology 9; 1, 137-143Hollinshead F.K., Walker, C. and Hanlon D. W. (2016) Determination of the normal reference interval for Anti-Müllerian Hormone (AMH) in bitches and use of AMH as a potential predictor of litter size. Reprod. Dom. Anim. 51; 3, 1-6Hollinshead F.K. and Krekeler N. (2016) ‘Feline Pyometra: to spay or not to spay’. Journal of Feline Medicine and Surgery 18; 1, 21-33Klewitz,J., Struebing, C., Rohn, K., Goergens, A., Martinsson, G., Orgies, F., Probst, J., Hollinshead, F., Bollwein, H. and Sieme, H. (2015) Effects of age, parity, and pregnancy abnormalities on foal birth weight and uterine blood flow in the mare Theriogenology 83, 721-729Malik, R., Howe, P. and Hollinshead, F. (2011) Deslorelin Implants - A New Choice in Feline Reproductive Medicine. Journal of Feline Medicine and Surgery 13, 874-875Hollinshead, F. K., Hanlon, D. W., Gilbert R. O., Krekeler N. and Volkmann, D. H. (2010) Calcium, parathyroid hormone, oxytocin and venous pH profiles in the whelping bitch. Theriogenology 73, 1276-1283Morton, K. M., Catt, S. L, Hollinshead, F. K., Maxwell, W. M. C. and Evans, G. (2005). The effect of gamete co-incubation time during in vitro fertilisation with frozen-thawed unsorted and sex-sorted spermatozoa on the development of in vitro matured adult and prepubertal ewe oocytes. Theriogenology 64, 363-377O’Brien, J. K., Stojanov, T., Heffernan S. J., Hollinshead, F. K., Vogelnest, L., Maxwell, W. M.C. and Evans, G. (2005). Flow cytometric sorting of non-human primate sperm nuclei. Theriogenology 63, 246-259Morton, K. M., Catt, S. L., Hollinshead, F. K., Maxwell, W. M. C. and Evans, G. (2004). Production of lambs after the transfer of fresh and cryopreserved in vitro produced embryos from prepubertal lamb oocytes and unsorted and sex-sorted frozen-thawed spermatozoa. Reprod. Dom. Anim. 39, 454-461Evans, G., Hollinshead, F. K. and Maxwell, W. M. C. (2004) Preservation and artificial insemination of sexed semen. Reprod. Fertil. Dev. 16, 455-464Maxwell, W. M. C., Evans, G., Hollinshead, F. K., Morton, K., Bathgate, R., De Graaf, S. P., Eriksson, B., Gillan, L., Morton, K. and O’Brien, J. K. (2004) Integration of sperm sexing technology into the ART toolbox. Anim. Reprod. Sci. 82-83, 79-95Hollinshead, F. K., O’Brien, J. K., Maxwell, W. M. C and Evans, G. (2004). Assessment of in vitro sperm characteristics after flow cytometric sorting of frozen-thawed bull spermatozoa. Theriogenology 62, 958-968

Research Specialty

Small animal (predominantly), equine, exotic/wildlifeResearch InterestsBreeding management and performance of large-scale working dog colonies; male and female infertility, ultrasonography during gestation in the bitch, semen biology and optimization of canine semen cryopreservation, contraception in small animals, isolation and characterization of equine and canine extracellular vesicles/exosomes for potential future therapeutic use for fertility and osteoarthritis, 3-D cell culture models of oviductal and endometrial organoids to study disease.Clinical ExpertiseCanine and feline reproduction and artificial reproductive technologies