Rushika Perera

Faculty Microbiology, Immunology, and Pathology

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            [ProfileText] => Dr. Perera is an Assistant Professor of RNA virology. Her laboratory is focused on studying the impact of cellular metabolism on Arbovirus replication within their vertebrate hosts and Arthropod vectors. She uses a systems biology approach (metabolomics/proteomics) combined with molecular virology, cell and structural biology, and biochemistry to study virus-host interactions. These viruses cause significant perturbations to the host metabolome to facilitate the formation of replication factories within specialized membrane structures. These structures are required for viral genome replication, assembly and egress. The host metabolome also fulfills the energy requirements for virus replication. Therefore, it is a key avenue to understand the dynamics of virus-host interactions. The goal of her research is to identify control points in cellular metabolic pathways that are required for virus replication, and evaluate these control points as novel targets for antiviral intervention.
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About Rushika

Dr. Perera is an Assistant Professor of RNA virology. Her laboratory is focused on studying the impact of cellular metabolism on Arbovirus replication within their vertebrate hosts and Arthropod vectors. She uses a systems biology approach (metabolomics/proteomics) combined with molecular virology, cell and structural biology, and biochemistry to study virus-host interactions. These viruses cause significant perturbations to the host metabolome to facilitate the formation of replication factories within specialized membrane structures. These structures are required for viral genome replication, assembly and egress. The host metabolome also fulfills the energy requirements for virus replication. Therefore, it is a key avenue to understand the dynamics of virus-host interactions. The goal of her research is to identify control points in cellular metabolic pathways that are required for virus replication, and evaluate these control points as novel targets for antiviral intervention.

Education

B.A., Goshen College, 1995Ph.D., Purdue University, 2002

Publications

Junjhon, J., Edwards, T. J., Utaipat, U., Bowman, V.D., Holdaway, H.A., Zhang, W., Keelapang, P., Puttikhunt, C., Perera R., Chipman, P.R., Kasinrerk, W., Malasit, P., Kuhn R.J. & Sittisombut, N. (2010). Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles. J Virol 84, 8353-8358. PMCID: PMC2916513Heaton, N. S., Perera, R., Berger, K. L., Khadka, S., Lacount, D. J., Kuhn, R. J. & Randall, G. (2010). Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and increases cellular fatty acid synthesis. Proc Natl Acad Sci U S A 107, 17345-17350. PMCID: PMC2951450Khadka, S., Vangeloff, A. D., Zhang, C., Siddavantam, P., Heaton, N., Sengupta, R., Hughes, R.E., Bell, R., Sahasrabudhe, S., Randall, G., Gribskov, M., Kuhn, R.J., Perera, R., & Lacount, D.J. (2011). A physical interaction network of dengue virus and human proteins. Molecular and Cellular Proteomics 10(12):M111.012187. PMCID: PMC3237087Plevka P., Perera R., Cardosa J., Kuhn R.J. and Rossmann M.R. (2012). Crystal structure of human enterovirus 71. Science Jun 8;336(6086):1274. PMCID: PMC3448362Perera, R., Riley, C., Hopf-Jannasch, Mezengie, G. I., A., Metz, T. O., Moore, R., Weitz, K. W., Pasa-Tolic, L., Adamec, J., & Kuhn, R.J. Dengue virus perturbs lipid metabolism in infected mosquito cells. Plos Pathogens. 8(3):e1002584. PMCID: PMC3310792Plevka P, Perera R, Yap ML, Cardosa J, Kuhn RJ, Rossmann MG. (2013). Structure of human enterovirus 71 in complex with a capsid-binding inhibitor. PNAS. PMCID: PMC3619292Plevka P, Perera R, Yap ML, Cardosa J, Kuhn RJ, Rossmann MG. (2013). Structure of human enterovirus 71 in complex with a capsid-binding inhibitor. Proc Natl Acad Sci U S A. Apr 2;110(14):5463-7. PMCID: PMC3619292Juhnjon J, Pennington, JG, Edwards TJ, Perera R, Lanman J and Richard J. Kuhn. (2014). Ultrastructural characterization and three-dimensional architecture of replication sites in dengue virus-infected mosquito cells. Journal of Virology. 88(9):4687-97

                
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