Ahmed Gad

Biomedical Sciences

About Ahmed

Ahmed Gad is a Post-doc fellow at the Animal Reproduction and Biotechnology Laboratory (ARBL), at Colorado State University. He got his Ph.D. in 2012 from the University of Bonn, Germany in the field of Animal Science and Biotechnology. Dr. Gad is currently working in the field of Reproductive Physiology and Embryo Genomics. His research is focused on the molecular mechanisms and pathways controlling oocyte and embryo development in different mammalian species.


Postdoctoral, Institute of Animal Physiology and Genetics, Czech Academy of Science, Libechov, Czech Republic. , 2017PhD, Institute of Animal Sciences, University of Bonn, Germany, 2012M.Sc., Faculty of Agriculture, Cairo University, Egypt., 2006B.Sc., Faculty of Agriculture, Cairo University, Giza, Egypt, 1999


Gad A; Murin M; Bartkova A; Kinterova V; Marcollova K; Laurincik J; Prochazka R (2022) Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality. Journal of Animal Science and Biotechnology 13:82Radek Procházka, Alexandra Bartková, Lucie Nemcová, Matej Murín, Ahmed Gad, Katerina Marcollová, Veronika Kinterová and Jozef Laurincík (2021) The role of MAPK3/1 and AKT in the acquisition of high meiotic and developmental competence of porcine oocytes cultured in vitro in FLI medium. International Journal of Molecular Sciences 22: 11148 Faheem M, Ghanem N, Gad A, Procházka R, Dessouki S (2021) Adaptive and biological responses of buffalo granulosa cells exposed to heat stress under in vitro condition. Animals 11 (3): 794Ghanem N, Ahmed D, Desouki S, Faheem M, Gad A, Peippo J, Barkawi A (2021): Cellular and molecular alterations of buffalo oocytes cultured under two different levels of oxygen tension during in vitro maturation. Zygote, 1-11.Saadeldin I, Gad A and Mermillod P (2021) Editorial: Biofluid Extracellular Vesicles and Their Involvement in Animal Reproductive Physiology. Front. Vet. Sci 8:747138Benc M, Strejcek F, Morovic M, Bartkova A, Murin M, Gad A, Bonnet-Garnier A, Popovska-Percinic F, Laurincik J (2021) Improving the quality of oocytes with the help of nucleolotransfer therapy. Pharmaceuticals 14 (4), 328 Gad A, Murín M, Nemcova L, Bartkova A; Laurincik J, Procházka R (2020): Inhibition of miR-152 during in vitro maturation enhances the developmental potential of porcine embryos. Animals 10 (12): 2289.Gad A, Sánchez JM, Browne J, Nemcova L, Laurincik J, Prochazka R, Lonergan P (2020) Plasma extracellular vesicle miRNAs as potential biomarkers of superstimulatory response in cattle. Scientific Reports 10: 19130, 1-16.Gebremedhn S, Gad A, Aglan H, Laurincik J, Prochazka R, Salilew-Wondim D, Hoelker M, Schellander K, Tesfaye D (2020) Extracellular vesicles shuttle protective messages against heat stress in bovine granulosa cells. Scientific Reports 10: 15824, 1-19. Koncicka M, Cervenka J, Jahn D, Sucha R, Vodicka P, Gad A, Alsheimer M, Susor A (2020) Expression of lamin C2 in mammalian oocytes. Plos One 15(4):e0229781.Gad A., Nemcova L., Murin M., Kinterova V., Kanka J., Laurincik J., Benc M., Pendovski L., Prochazka R. (2020) Global transcriptome analysis of porcine oocytes in correlation with follicle size. Molecular Reproduction and Development 87 (1): 102-114 Ahmed D, Ghanem N, Dessouki S, Faheem M, Gad A and Barkawi A (2020) Developmental Competence of Buffalo Oocytes Cultured Under Different Oxygen Tensions after Selection with Brilliant Cresyl Blue. World’s Veterinary Journal 10 (2): 256-263Ashour G, Gad A, Fayed A.K, Ashmawy N, El- Sayed A (2020): Evaluation of growth performance, blood metabolites and gene expression analysis in Egyptian sheep breeds in relation to age. World’s Veterinary Journal 10 (1): 18-29 Gebremedhn S, Ali A, Gad A, Procházka R, Tesfaye D (2020): Extracellular Vesicles as Mediators of Environmental and Metabolic stress Coping Mechanisms during Mammalian Follicular Development. Front. Vet. Sci. 7: 961 Gad A., Nemcova L., Murin M., Kanka J., Laurincik J., Benc M., Pendovski L., Prochazka R. (2019) MicroRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles. Molecular Reproduction and Development 86 (4):426–439.Sakr O., Gad A.,?Rodríguez M., Rebollar P., Millánc P. (2019) Superoxide dismutase mimics improve semen quality during chilled preservation of rabbit spermatozoa. Livestock Science. 221: 70-76 Gad, A., S. Abu Hamed, M. Khalifa, A. Amin, A. El-Sayed, S. A. Swiefy, and S. El-Assal (2018) Retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes in in vitro matured buffalo (Bubalus bubalis) oocytes. International Journal of Veterinary Science and Medicine. 6(2): 279–285.Abdelatty A.M., Iwaniuk M.E., Potts S.B., Gad A (2018) Influence of Maternal Nutrition and Heat Stress on Bovine Oocyte and Embryo Development. International Journal of Veterinary Science and Medicine 6: S1-S5. El-Rashidy AA,?Gad A,?Abu-Hussein AE,?Habib SI,?Badr NA,?Hashem AA (2015) Chemical and Biological Evaluation of Egyptian Nile Tilapia (Oreochromis Niloticas) Fish Scale Collagen. Int J Biol Macromol 79:618-626El-Sayed A., Ashour G., Khalifa M., Gad A. (2015) Vitrification assessment of buffalo (Bubalus bubalis) oocytes: morphological and molecular aspects. Egyptian J. Anim. Prod. 52: 1-6 El-Rashidy AA,?Hashem AA,?Badr NA, Habib SI,??Gad A,?Abu-Hussein AE, (2015) Extraction and characterization of collagen type (1) from Nile Tilapia fish scales. Egyptian Dental Journal 61:327-332 Amin A, Gad A, Salilew-Wondim D, Prastowo S, Held E, Hoelker M, Rings F, Tholen E, Neuhoff C, Looft C, Schellander K, Tesfaye D (2014) Bovine embryo survival under oxidative-stress conditions is associated with activity of the NRF2-mediated oxidative-stress-response pathway. Molecular Reproduction & Development 81:497–513 Gunawan A, Sahadevan S, Neuhoff C, Große-Brinkhaus C, Gad A, et al. (2013) RNA Deep Sequencing Reveals Novel Candidate Genes and Polymorphisms in Boar Testis and Liver Tissues with Divergent Androstenone Levels. PLoS ONE 8(5): e63259.Abd El Naby WS, Hagos TH, Hossain MM, Salilew-Wondim D, Gad AY, Rings F, Cinar MU, Tholen E, Looft C, Schellander K, Hoelker M and Tesfaye D?(2013) Expression analysis of regulatory microRNAs in bovine cumulus oocyte complex and preimplantation embryos. Zygote 11, 1-21. Forde N,?Carter F,?di Francesco S,?Prakash Mehta J,?Garcia-Herreros M,?Gad AY,?Tesfaye D,?Hoelker M,?Schellander K,?Lonergan P (2012) Endometrial response of beef heifers on Day 7 following insemination to supra-physiological concentrations of progesterone associated with superovulation. Physiol Genomics. 44 (22): 1107-15. Gad A, Hölker M, Besenfelder U, Havlicek V, Cinar U, Rings F, Held E, Dufort I, Sirard MA, Schellander K, Tesfaye D (2012) Molecular Mechanisms and Pathways Involved in Bovine Embryonic Genome Activation and Their Regulation by Alternative In vivo and In vitro Culture Conditions. Biology of Reproduction 87 (4) 1-13.Gad A, Tesfaye D, Schellander K (2012) Transcriptome profile of early mammalian embryos in response to culture environment. Anim. Reprod. Sci. 134: 76-83.Abdoon AS, Ghanem N, Kandil OM, Gad A, Schellander K, Tesfaye D (2012) cDNA microarray analysis of gene expression in parthenotes and in vitro produced buffalo embryos. Theriogenology 77, 1240-1251. Gad A, Besenfelder U, Rings F, Ghanem N, Salilew-Wondim D, Hossain MM, Tesfaye D, Lonergan P, Becker A, Cinar U, Schellander K, Havlicek V, Hölker M (2011) Effect of reproductive tract environment following controlled ovarian hyperstimulation treatment on embryo development and global transcriptome profile of blastocysts: implications for animal breeding and human assisted reproduction. Human Reproduction 26, 1693-1707. Ghanem N, Salilew Wondim D, Tesfaye D, Gad A, Phatsara C, Tholen E, Looft C, Schellander K, Hoelker M (2011) Bovine blastocysts with developmental competence to term share similar expression of developmentally important genes although derived from different culture environments. Reproduction 142, 551-564. El-Gendy EA, Gad AY and Mostageer A (2006) Sperm-mediated gene transfer in poultry 1. The relationship with cock sperm viability. Arab Journal of Biotechnology 10: 1-12.

Research Specialty

Reproductive PhysiologyEmbryo Genomics