Serum Follicle Stimulating Hormone (oFSH, eFSH, rFSH, bFSH)

Type of hormone: Protein

Purpose of Test: To determine serum concentration of FSH in order to obtain information about reproductive function. Reasons to perform a FSH test vary with the species and include:

• Check for neuter/spay status

• Aid in diagnosis of reproductive disorders

Description of test and procedures: The test is a radioimmunoassay performed with in-house reagents. The radioimmunoassay technique is based on the competitive binding of the serum FSH and a radiolabeled FSH preparation. They compete for binding to an antibody specific for FSH. The antibody-bound radiolabeled FSH is separated, and the quantity is determined by counting on a gamma spectrometer. Results for the unknown are read from a curve prepared by plotting results for a set of known standards. Serum with a pre-determined concentrations are included in the assay for quality control purposes.

Sample needed and procedures for submittal: Submit at least 1mL of serum on a cold pack. It may be possible to perform this analysis with less serum, but laboratory approval is required.

Interpretation of results: Results should be interpreted by someone familiar with reproductive function in the species being tested.